BIOL 1202 lab study assignments and lab guidesheets
For each week's assigned lab work you must print a copy
of the appropriate lab guidesheet(s), shown as links below. You must
bring the guidesheet(s) to lab; it contains the instructions you need to do the
lab work. Note that for some weeks' assigned work, there may be more than
one guidesheet to print.
For week #7 in lab (Mon. 3/5 – Fri. 3/9)
1. Study the detailed guidesheet entitled "More about Proteins and Lab Technique." Print it and bring it to lab; it is your set of instructions. This lab work continues the work you did in the previous exercise. So, bring to lab also the guidesheet from week #6; it contains the instructions for preparation of the enzyme extract and the assay procedures.
3. Study the material about properties of enzymes in the text (Chap. 8) and in your lecture notes. Concentrate especially on factors that influence rates of enzyme activity, in particular the effects of pH, temperature, organic solvents, metabolic inhibitors (irreversible, reversible, competitive; pp. 161-164).
4. Since you will be using the Spectronic 20 colorimeter as a tool to track the progress of the reaction that the peroxidase enzyme catalyzes, you should review the operation of that instrument and the principles of colorimetry, which you studied and applied three times already. You will need to know how to standardize the instrument.
5. Bring your calculator. Graph paper isn't needed.