SETUP TABLE, part 2 assays #9-17 (effects of heat, organic solvent, and metabolic inhibitors on reaction rate)
Assay #	mL 0.01 M guaiacol	mL water	mL 0.1 % (v/v) H2O2	Treatment*	mL enzyme extract	time (seconds)
9	2.0	2.0	0.8	100 C, 60 seconds (See part IV instructions)	0.2
10	2.0	1.0	0.8	1.0 mL ethanol (See part V instructions)	0.2
#11-13 are a set: effect of an irreversible inhibitor. See part VI instructions.
11	0.1	3.3	1.4	none	0.2
12	0.1	3.1	1.4	0.2 mL NaF	0.2
13	0.2	3.0	1.4	0.2 mL NaF	0.2
#14-17 are a set: effect of a reversible (and competitive) inhibitor. See part VII instructions.
14	0.1	3.3	1.4	none	0.2
15	0.1	3.2	1.4	0.1 mL catechol	0.2
16	0.2	3.1	1.4	0.1 mL catechol	0.2
17	0.0	3.3	1.4	0.1 mL catechol	0.2
* Ethanol is 95 % (v/v). NaF stock is 10^-1 M. Catechol stock is 5 X 10^-3 M.