SETUP TABLE, part 1

assays #2-8 (effect of pH on reaction rate)

 

Assay #

mL

0.01 M

guaiacol

 

mL

water

mL

0.1 % (v/v)

H2O2

 

HCl**

mL

enzyme

extract

time

(seconds)

1 *

(blank)

2.0

2.2

0.8

none

0.0

not applicable

2

2.0

0.0

0.8

2. 0 mL of

2.5 X 10-7 M

0.2

 

3

2.0

0.0

0.8

2.0 mL of

2.5 X 10-6 M

0.2

 

4

2.0

0.0

0.8

2.0 mL of

2.5 X 10-5 M

0.2

 

5

2.0

0.0

0.8

2.0 mL of

2.5 X 10-4 M

0.2

 

6

2.0

0.0

0.8

2.0 mL of

2.5 X 10-3 M

0.2

 

7

2.0

0.0

0.8

2.0 mL of

2.5 X 10-2 M

0.2

 

8

2.0

1.6

0.8

See part III.C. instructions.

0.2

 

* No enzyme, no reaction; therefore no color development.  Use #1 to standardize the colorimeter.

** These HCl solutions are prepared, prior to doing assays, by serial dilution of a

2.5 X 10-1 M HCl solution: see part III.A instructions.