SETUP TABLE, part 1 assays #2-8 (effect of pH on reaction rate) |
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Assay # |
mL 0.01 M guaiacol |
mL water |
mL 0.1 % (v/v) H2O2 |
HCl** |
mL enzyme extract |
time (seconds) |
1 * (blank) |
2.0 |
2.2 |
0.8 |
none |
0.0 |
not applicable |
2 |
2.0 |
0.0 |
0.8 |
2. 0 mL of 2.5 X 10-7 M |
0.2 |
|
3 |
2.0 |
0.0 |
0.8 |
2.0 mL of 2.5 X 10-6 M |
0.2 |
|
4 |
2.0 |
0.0 |
0.8 |
2.0 mL of 2.5 X 10-5 M |
0.2 |
|
5 |
2.0 |
0.0 |
0.8 |
2.0 mL of 2.5 X 10-4 M |
0.2 |
|
6 |
2.0 |
0.0 |
0.8 |
2.0 mL of 2.5 X 10-3 M |
0.2 |
|
7 |
2.0 |
0.0 |
0.8 |
2.0 mL of 2.5 X 10-2 M |
0.2 |
|
8 |
2.0 |
1.6 |
0.8 |
See part III.C. instructions. |
0.2 |
|
* No enzyme, no reaction; therefore no color development. Use #1 to standardize the colorimeter. |
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** These HCl solutions are prepared, prior to doing assays, by serial dilution of a 2.5 X 10-1 M HCl solution: see part III.A instructions. |