BIOL 1202 lab study assignments and lab guidesheets
For each week's assigned lab work you must print a copy of the appropriate lab guidesheet(s), shown as links below. You must bring the guidesheet(s) to lab; it contains the instructions you need to do the lab work. Note that for some weeks' assigned work, there may be more than one guidesheet to print.
For week #10 in lab (Wed. April 11 – Tue. April 17)
The topic is electrophoresis of proteins.
1. Review in your lecture notes and in the textbook the material about (a) protein structure, (b) charges on functional groups, amino acids and protein molecules, (c) effect of pH on charged character of protein molecules
2. Study the lab guidesheet, entitled ELECTROPHORESIS and the two pages of related figures (Figs1-4, Figs5-8). The guidesheet gives background information and instructions for the lab work. Bring the guidesheet and the figures to lab.
3. Electrophoresis is a technique that can be used to separate charged molecules from each other, notably proteins and nucleic acids. In the case of the latter, it can separate DNA fragments from each other, which lead to its application in DNA "fingerprinting" and other uses in biotechnology. You can read about such an application in the textbook, pp. 324-326 (with illustrations).