SETUP TABLE, part 2
assays #13-15 (effect of enzyme concentration on reaction rate)
assays #16-18 (reproducibility of results)
Assay #
mL
0.01 M
guaiacol
mL
water
mL
0.1% (v/v)
H2O2
mL
enzyme
extract
time
(seconds)
[The blank, assay #1, is the same for these as for #2-12; the colorimeter is already standardized.]
13*
2.0
1.4
1.2
0.4
  
14*
2.0
1.6
1.2
0.2
  
15*
2.0
1.7
1.2
0.1
  
16**
2.0
2.0
0.8
0.2
  
17**
2.0
2.0
0.8
0.2
  
18**
2.0
2.0
0.8
0.2
  
* Concentration of both substrates is held constant in all 3 assays while enzyme concentration is changed.
** All 3 assays are identical in composition, same as assay #5. But your measuring, mixing, reading may vary from one to another. So these 3 assays check the effect of those factors on results.