SETUP TABLE, part 1
assays #2-12 (effect of substrate concentration on reaction rate)
Assay #
mL
0.01 M
guaiacol
mL
water**
mL
0.1% (v/v)
H2O2**
mL
enzyme
extract
time
(seconds)
1* 
(blank)
2.0
2.2
0.8
0.0
not 
applicable
2
2.0
0.8
2.0
0.2
  
3
2.0
1.2
1.6
0.2
  
4
2.0
1.6
1.2
0.2
  
5
2.0
2.0
0.8
0.2
  
6
2.0
2.2
0.6
0.2
  
7
2.0
2.4
0.4
0.2
  
8
2.0
2.5
0.3
0.2
  
9
2.0
2.6
0.2
0.2
  
10
2.0
2.7
0.1
0.2
  
11
2.0
2.75
0.05
0.2
  
12
2.0
2.8
0.0
0.2
  
* No enzyme, no reaction; therefore, no color development. Use #1 to standardize colorimeter.
**Total assay volume = 5 mL; all solutions are aqueous. The change in H2O2 volume from one assay to the next is compensated by H2O to keep the volume of H2O2 plus the volume of H2O = 2.8 mL in #2-12.. The concentration of the other substrate, guaiacol, is constant.