Fall 2002 


INSTRUCTOR: Dr. Carolyn Bentivegna

Telephone: 973-275-2113
McNulty Hall Room #208

OFFICE HOURS: M, W  10-12 or by appointment


TEXTBOOK: Modern Genetic Analysis: Integrating Genes and Genomes, A.J.F Griffiths, W.M. Gelbart, R.C. Lewontin and J.H. Miller, 2nd edition, W.H. Freeman and Co., NY.

The purpose of this course is to give a good overview of genetics. Classic theory such as Mendelian principles, chromosomal mapping, population genetics and DNA structure and function will be covered.  In addition, modern genomics including gene expression control and tools for DNA recombination will be presented.  Laboratory emphasis will be on techniques for genome analysis such as bacterial cloning, PCR, and restriction enzyme digestion.

9/4 Introduction Chp. 1-
9/6  DNA Chemistry/Chromosomes Chp. 2
9/9  Gene Function/Transcription Chp. 3
9/11  Gene Function/Translation Chp. 3
9/13  DNA Replication/Homework 1 due Chp. 4
9/16 Mitosis and Meiosis Chp. 4
9/18 Mendelian Principles: Mono- and Dihybrid Crosses Chp. 5
9/20  Pedigree Analysis Chp. 5
9/23 Review/ Homework 2 due -
9/25 Exam I -
9/27 Genetic Recombination and Mapping in Eukaryotes Chp. 6
9/30 Genetic Recombination and Mapping in Eukaryotes Chp. 6
10/2  Genetic Recombination and Chi Square in Eukaryotes Chp. 6
10/4 Genetic Recombination and Mapping in Prokaryotes/Homework 3  due Chp. 7
10/7 Genetic Recombination and Mapping in Prokaryotes -
10/9 Recombinant DNA Tools Chp. 7
10/11 Fall Break, No Classes Chp. 8
10/14 PCR and DNA sequencing Chp. 8, 9
10/16 Restriction Mapping Chp 8
10/18 Review/Homework 4 due -
10/21 Exam II -
10/23 Gene Expression Control in Prokaryotes Chp. 13
10/25 Gene Expression Control in Prokaryotes Chp. 13
10/28 Gene Expression Control in Eukaryotes Chp. 13
10/30 Gene Expression Control in Eukaryotes/Homework 5 due Chp. 13
11/1 DNA mutation Chp. 10
11/4 DNA mutation Chp. 10
11/6 Chromosome Mutation Chp. 11
11/8 Chromosome Mutation Chp. 11
11/11 Review/Homework 6 due -
11/13 Exam III -
11/15 Gene Interaction Chp. 14
11/18 SETAC Meeting, No Class Chp. 16
11/20 SETAC Meeting, No Class Chp. 16
11/22 Gene Interaction Chp. 14
11/25 Genetic Basis of Development Chp. 16
11/27-29 No Class, Happy Thanksgiving and Happy Hanukah -
12/2 Genetic Basis of Development/Homework 7 due Chp. 16
12/4 Population Genetics: Hardy-Weinberg Chp. 17
12/6 Population Genetics: Processes that Change Allelic Frequencies Chp. 17
12/9 Evolution and Speciation Chp. 19
12/11 Evolution and Speciation Chp. 19
12/13 Review/Homework 8 due -
12/16 Exam IV, 2:45-4:25 PM -
-Course content and schedule of assignments are subject to change.

    Point distribution will be as follows:
Assignment Points
Total points
Exam I 150  
1000-900 (100-90%) 
Exam II 150 
899-850 (89-85%)
Exam III 150 
849-800 (84-80%)
Exam IV 150 
799-750 (79-75%)
Laboratory Exam I 100 
749-680 (74-68%)
Laboratory Exam II 100 
679-600 (67-60%)
Lab Notebook (11 x 10) 110 
599-550 (59-55%)
Homework (7 x 12) + (1 x 6) 90
549-0 (54-0%)

Week of Laboratory Assignment
9/9 Chromosome squashing/ pipetting Notebook 1
9/16 DNA isolation Notebook 2
9/23 DNA quantification and PCR Notebook 3
9/30 Gel electrophoresis/band isolation/PCR video Notebook 4
10/7 Maize mono/dihybrid crosses Notebook 5
10/14 Bacterial plasmid ligation Laboratory Exam I
10/21 Bacterial transformation Notebook 6
10/28 Analysis of bacterial transformation/PCR Notebook 7
11/4 Gel electrophoresis/band isolation Notebook 8
11/11 Karyotyping Notebook 9
11/18 Restriction Enzyme Digest/Gel electrophoresis Notebook 10
11/25 No lab -
12/2 RE Map and BLAST analysis Notebook 11
12/9   Laboratory Exam II

Lecture Exams
    There will be four lecture exams, each worth 150 points.  Make-up exams will only be given if I am contacted ahead of time and provided with a doctors note when appropriate.  Cheating on an exam will result in zero credit for that exam.  Exams are not cumulative.


    The laboratory grade will be based on two exams and a notebook.  You must attend your assigned laboratory section.  If you have a legitimate excuse (requires a note), you must arrange with me to attend another section.  TA's can not arrange for you to attend another lab section.  Once a particular laboratory is finished for the week, it can not be made up.  So think ahead.  Notebooks will be collected at the end of each lab session and graded for that session.  If you miss a lab, you will loose all the points for that week's lab.
    Laboratory exams are worth 100 points each.  They will be based on knowledge of theory and practical skills presented in lab sessions.  Exams are not cumulative.  If you have a legitimate excuse (requires a note) for missing a laboratory exam, it can be made up in another section with my permission (not a TA's permission).  Lab exams can not be made up after the week they are scheduled. So think ahead.
    Laboratory notebooks are worth 10 points per lab for a total of 110 points.  Components of the notebook include 1) introduction, 2) methods, 3) results and 4) conclusion.  If all components are not finished at the end of a lab session, notebooks will be graded on what should have been completed for that session.  Details will be given in lab handouts.  Students may work together on laboratory projects, but each student must hand in their own notebook written in their own words.

    Homework will be assigned worth 12 or 6 points each not a total of 90 points.  The type of assignment will depend on the current lecture topic and include, for example, problem sets, data analysis and literature review.  They are due on the designated date.  One point will be subtract for each day they are late.  Plagiarism will result in zero credit for that assignment.

I am happy to help you with this course. Please, do not hesitate to contact me.  Your teaching assistants are also available for help.  Please contact them in person or through e-mail.